maxiscript t7 transcription kit am1312 (Thermo Fisher)

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Thermo Fisher maxiscript t7 transcription kit am1312
Reagents and tools table

Maxiscript T7 Transcription Kit Am1312, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/maxiscript+t7+transcription+kit+am1312/maxiscript+t7+transcription+kit/pmc11962161-57-9-5
Average 90 stars, based on 1 article reviews

maxiscript t7 transcription kit am1312 - by Bioz Stars, 2026-09

90/100 stars

Images

1) Product Images from "Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processing"

Article Title: Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processing

Journal: The EMBO Journal

doi: 10.1038/s44318-025-00394-4

Figure Legend Snippet: Reagents and tools table

Techniques Used: Recombinant, Plasmid Preparation, Sequencing, Protease Inhibitor, SYBR Green Assay, Reverse Transcription, Immunoprecipitation, Software

Synthesized:

Article Title: Rixosomal RNA degradation contributes to silencing of Polycomb target genes.
Article Snippet: All the siRNAs were synthesized by Dharmacon and are listed in Supplementary Table 1. .. Small guide RNA was synthesized via in vitro transcription by using MAXIscript T7 transcription kit (ThermoFisher, AM1312). ..

Article Title: Genetically defined nucleus incertus neurons differ in connectivity and function
Article Snippet: The resulting DNA was cloned into the pDR274 vector Addgene, plasmid #42250; following digestion of pDR274 with Bsa I (R3733S, New England Biolabs). .. The pDR274 templates and the pDR274 vector for synthesis of the GFP bait sgRNA ( ) were digested by Dra I and sgRNAs synthesized using the MAXIscript T7 Transcription Kit (AM1312, Thermo Fisher Scientific). pT3TS-nCas9n template DNA (Addgene, plasmid #46757; ) was digested with Xba I (R0145S, New England Biolabs), and Cas9 RNA was synthesized using the mMESSAGE mMACHINE Transcription Kit (AM1348, Thermo Fisher Scientific). ..

Article Title: CDK11 regulates pre-mRNA splicing by phosphorylation of SF3B1.
Article Snippet: .. Uniformly [32P]-labelled AdML pre-mRNA was synthesized in vitro using the MAXIscript T7 Transcription Kit (Thermo Fisher Scientific, AM1312) using [α-32P]UTP (Hartmann Analytic, FP-210), purified from 15% acrylamide gel containing 6 M urea and followed by phenol extraction. .. Splicing reactions contained 40% HeLa nuclear extract (Ipracell, CC-01-20-005; stock concentration 6 mg ml−1 in 20 mM HEPES-KOH (pH 7.9), 100 mM KCl, 0.2 mM EDTA, 20% glycerol, 0.2 mM PMSF and 0.5 mM DTT) supplemented with KCl to 80 mM final concentration, 1 U of SUPERase•In RNase Inhibitor (20 U μl−1, Thermo Fisher Scientific, AM2694), 3 mM MgCl2, 1 mM ATP, 10 mM creatine phosphate and 4 ng of [ 32P]-labelled AdML pre-mRNA in 20 μl.

In Vitro:

Article Title: Zebrafish Cyp1b1 knockout alters eye and brain metabolomic profiles, affecting ocular and neurobehavioral function.
Article Snippet: Cytochrome P450 1B1 (CYP1B1) metabolizes endogenous and xenobiotic substrates, including steroids and fatty acids.. It is implicated in the metabolism of compounds essential for eye development and is a causative gene in primary congenital glaucoma (PCG).. However, CYP1B1's role in PCG and related eye disorders and neurobehavioral function is poorly understood.

other:

Article Title: Modular in vivo assembly of Arabidopsis FCA oligomers into condensates competent for RNA 3’ processing
Article Snippet: MAXIscript T7 Transcription Kit , Thermo Fisher Scientific , AM1312.

Purification:

Polymerase Chain Reaction:

Plasmid Preparation:

Acrylamide Gel Assay:

Extraction:

Marker:

Article Title: DNA-directed termination of RNA polymerase II transcription.
Article Snippet: .. Fluorescently labelled RNA marker was produced by incorporating Fluorescein-12-UTP (Jena bioscience, NU-821-FAMX) into RNA marker template (Thermo Fisher Scientific, AM7782) via the MAXIscript T7 transcription kit (Thermo Fisher Scientific, AM1312). .. In vitro transcription and termination The elongation complexwas assembled as previously describedwithminormodification.87 Briefly, 5 pmol of pre-annealed RNA:DNA (template strand) hybrid was mixed with an equimolar amount of pure RNAPII, followed by the addition of 10 pmol 5’ biotin-labelled non-template strand DNA, and incubation.87 The assembled elongation complex was then immobilized onto streptavidin beads T1 (Thermo Fisher Scientific, 65602) and washed with transcription buffer (TB) containing 20 mM Tris-HCl pH 7.5, 100 mM NaCl, 8 mM MgCl2, 10 mMZnCl2, 10%glycerol, 2 mMDTT, then with TB/0.1% Triton, TB/0.5 MNaCl and finally TB buffer.

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